partec flowmax Search Results


flowmax software  (Partec)
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Partec flowmax software
Flowmax Software, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax cytometer pasiii  (Partec)
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Partec flowmax cytometer pasiii
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax Cytometer Pasiii, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax program  (Partec)
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Partec flowmax program
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax Program, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax program - by Bioz Stars, 2026-04
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partec flowmax® software  (Partec)
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Partec partec flowmax® software
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Partec Flowmax® Software, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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partec flowmax® software - by Bioz Stars, 2026-04
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acquisition software flowmax  (Partec)
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Partec acquisition software flowmax
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Acquisition Software Flowmax, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax  (Partec)
90
Partec flowmax
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax 2·4b (xp) software  (Partec)
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Partec flowmax 2·4b (xp) software
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax 2·4b (Xp) Software, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax facs machine  (Partec)
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Partec flowmax facs machine
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax Facs Machine, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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software flowmax version 2.4  (Partec)
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Partec software flowmax version 2.4
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Software Flowmax Version 2.4, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax fcm v2.4f  (Partec)
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Partec flowmax fcm v2.4f
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax Fcm V2.4f, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax 3.0 flow cytometer  (Partec)
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Partec flowmax 3.0 flow cytometer
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax 3.0 Flow Cytometer, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyflow space flowmax 2.8  (Partec)
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Partec cyflow space flowmax 2.8
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Cyflow Space Flowmax 2.8, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow cytometry during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.

Journal: Immunology

Article Title: Interleukin‐6 signalling mediates Galectin‐8 co‐stimulatory activity of antigen‐specific CD 4 T‐cell response

doi: 10.1111/imm.12980

Figure Lengend Snippet: Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow cytometry during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.

Article Snippet: Flow cytometry analysis FlowMax cytometer PASIII (Partec, Münster, Germany) and flowjo software (FlowJo, Ashland, OR, USA) were used throughout this work.

Techniques: Expressing, Flow Cytometry, Cell Culture, Fluorescence, Recombinant